Plasmid

Part:BBa_K5302016:Design

Designed by: Xinyuan Shi   Group: iGEM24_USTC   (2024-10-01)


pBBR-OmpA-VEGFR1D2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2382
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2382
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NotI site found at 5314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2382
    Illegal BglII site found at 6060
    Illegal BamHI site found at 1302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2382
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2382
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 3040
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 2880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5636
    Illegal SapI.rc site found at 423
    Illegal SapI.rc site found at 633


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

VEGFR1D2 is the second extracellular domain of human VEGFR1. OmpA (Outer Membrane Protein A) from Escherichia coli str. K-12 substr. MG1655.

References